It remains difficult to reach an anatomopathological diagnosis of dysplasia associated to inflammation (DAI) in UC, especially when tissue inflammation is present. The study by Merli et al. aimed to compare the proteomes of dysplastic, inflammatory, and normal paired tissues . Their approach highlighted specific proteins such as Solute Carrier Family 12 member 2 (SLC12A2) which may aid diagnosis of UC-DAI.
This small study was performed on formalin-fixed, paraffin-embedded samples from UC-DAI (n=5). Epithelial cells from the paired tissues were collected by laser capture microdissection and differentially analysed using label-free proteomics.
Proteomic analysis enabled confident identification of 1,070 proteins, among which 19 proteins showed differential abundance between DAI and inflammatory or normal tissue. Of these, SLC12A2 was only detected in DAI. Tissue distribution of SLC12A2 was assessed by immunohistochemistry on UC-DAI (n=11). Colon...
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