Home > Gastroenterology > ECCO 2020 > Miscellaneous Topics > Re-inducing inflammation in organoids from UC patients

Re-inducing inflammation in organoids from UC patients

Presented By
Dr K. Arnauts, Catholic University of Leuven, Belgium
Conference
ECCO 2020
Patient-derived intestinal organoids provide a powerful tool to better understand mechanisms underlying inflammatory bowel disease (IBD), but patient-specific organoids quickly lose their inflammatory phenotype. A Belgian group showed that inflammation can efficiently be (re-)induced in organoids from ulcerative colitis (UC) patients as well as non-IBD individuals [1]. First author Kaline Arnauts (Catholic University of Leuven, Belgium) explained the hypothesis that in order to study UC in an ex vivo model, inflammation should be re-induced towards levels corresponding to the in vivo situation. The researchers also wanted to know if organoids derived from inflamed regions are relatively sensitive to inflammatory stimulation. They therefore obtained biopsies from 8 patients with active UC, both from inflamed and non-inflamed regions, and from 8 non-IBD controls. Exposure to the inflammatory mix induced transcriptional activation of ...


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