Home > Dermatology > Whole-exome sequencing to study the underlying pathogenesis of psoriasis

Whole-exome sequencing to study the underlying pathogenesis of psoriasis

Presented By
Mr Sigurgeir Olafsson, University of Cambridge, UK
Conference
WPPAC 2021
Using laser-capture microdissection and subsequent whole-genome sequencing, Mr Sigurgeir Olafsson (University of Cambridge, UK) and colleagues were able to analyse skin biopsies from many patients with psoriasis vulgaris. This technique of whole-exome sequencing will improve understanding of the underlying pathogenesis of psoriasis vulgaris. Somatic mutations may contribute to the pathogenesis of complex diseases. Also, chronic inflammation can affect disease progression. Although the hyperproliferation of keratinocytes is associated with the disease status of psoriasis, no differences have been observed in the clonal composition of lesional and non-lesional epidermis. The current study aimed to investigate these differences. In this study, laser-capture microdissection was used to isolate 140 small samples of epidermis from 16 patient donors. Afterwards, these samples were assessed by whole-exome sequencing. In previous studies, hundreds of oncogen...


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